Antibody Sequencing for Phosphorylation Research
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INQUIRYCreative BioMart provides our customers with comprehensive and accurate antibody sequencing services to accelerate their kinase/phosphatase biology research and drug development programs. As a leading biotechnology company with many years of experience in antibody research, we have a team of highly skilled technicians who have accumulated extensive experience in antibody sequencing and are able to deliver high quality sequencing results in a short period of time.
Introduction to Antibody Sequencing
Antibodies are a vital part of the immune system and have been developed as a powerful research tool. Getting the sequence from an antibody is important for antibody engineering, function optimization, novel antibody discovery, and database banking. Antibodies with 100% accurate sequences can avoid many issues such as genetic drift and the loss of hybridoma cell line due to contamination. Antibodies sequencing can provide important information for various downstream applications, such as expression of recombinant antibodies, affinity maturation, antibody humanization, and antibody format alteration. Moreover, the unique sequence of amino acids in an antibody, especially variable region sequences, is the key determinant of antibody novelty and plays a crucial role in the patent application.
Figure 1. Approaches for high-throughput sequencing of functional antibody repertoires. (Robinson W H, 2015)
Featured Services
Creative BioMart offers a full package of antibody sequencing services to help our customers save time and cost. Our comprehensive services based on different antibody sequencing strategies will accelerate your journey to success. If you have any additional questions, please fill out the online inquiry form and let us know more details.
De Novo Antibody Sequencing
De novo antibody sequencing can be used to determine the amino acid sequence of an unknown antibody without prior knowledge of the DNA or protein sequence. Creative BioMart has developed proprietary technology for de novo antibody sequencing to achieve 100% coverage of the desired regions and excellent accuracy.
Hybridoma Sequencing
Hybridoma sequencing provides sequence information on the cDNA encoding the variable heavy (VH) and variable light (VL) domains of antibodies produced by hybridoma cell lines. Creative BioMart has established a high-accuracy and high-throughput hybridoma sequencing platform to offer a quick and professional service covering variable region and full-length antibody sequencing.
Antibody Repertoire Sequencing
The antibody repertoire refers to the entire set of antibodies produced by the immune system. Antibody repertoire sequencing may provide useful clues into the mechanisms of immune responses. Creative BioMart provides a high-throughput platform for large-scale antibody repertoires sequencing to accelerate downstream applications such as antibody discovery, biomarker discovery, and the diagnostic tool development.
Service Details
Creative BioMart provides antibody sequencing services covering diverse species, including but not limited to human, rabbit, rat, mouse, rodent, chicken, and sheep.
| Customer provides | Monoclonal antibody, polyclonal antibody, immunoglobulin (IgA, IgG, IgE, IgM), conjugated antibody, human/humanized antibody, single-domain antibody, biosimilar monoclonal antibody, or antibody-expressing hybridoma. |
| Timelines | Depends on the project details. |
| Deliverables | Comprehensive sequencing report coupled with QC data. |
Highlights
- Unparalleled accuracy, sensitivity, and flexibility.
- Wide applicability for all antibody types and species.
- Faster turnaround time supported by high-throughput techniques.
- Professional scientists responsible for quality control.
- Customized services to fulfill specific needs.
Creative BioMart has established a reliable and high-throughput antibody sequencing platform to provide top-quality services to assist our customers in antibody development and obtaining intellectual property rights for their antibody sequences. We also provide follow on services such as recombinant antibody production and antibody engineering. If you are interested in our services, please do not hesitate to contact us.
References
- Robinson W H. Sequencing the functional antibody repertoire—diagnostic and therapeutic discovery. Nature Reviews Rheumatology, 2015, 11(3): 171.
- Georgiou G, et al. The promise and challenge of high-throughput sequencing of the antibody repertoire. Nature biotechnology, 2014, 32(2): 158-168.