Custom Protein Production for Phosphorylation Research

Custom Protein Production for Phosphorylation Research

INQUIRY

Creative BioMart offers numerous options for custom protein production services to accelerate kinase/phosphatase biology research and pharmaceutical development. Our extensive experience in protein production allows us to deliver high-quality proteins and provide comprehensive services within a guaranteed time.

Background

As an essential part of living organisms, proteins play an important role in virtually every process within cells, such as cell signaling, cell cycle, cell adhesion, cell metabolism, and immune responses. The study of protein structure and function has become the focus of current research. Many proteins have become very attractive sources for therapeutic development due to their high intrinsic potency and selectivity for biological targets. The production of specific proteins is usually achieved by manipulating gene expression in an organism. It has been developed as a widely used biotechnological process in life sciences, biotechnology and medicine.

Protein phosphorylation is an extremely important regulatory mechanism in various cellular processes. Kinases and phosphatases are drivers of protein phosphorylation and dephosphorylation events. Production of kinases and phosphatases as well as their substrates is essential to study the mechanism of protein phosphorylation and the development of drugs targeting kinase/phosphatase signaling networks.

Figure 1. Schematic representation of hTLK1B-pETDUET-1-Lambda Protein Phosphatase expression vector (A) and coexpression of recombinant 6×His-tagged hTLK1B along with bacteriophage lambda protein phosphatase at 25 °C at different IPTG concentrations. (Bhoir S, et al., 2018)

Available Expression Systems

Both cell-based and cell-free systems are available at Creative BioMart. We provide our customers with maximum flexibility to meet their needs in the life sciences and pharmaceutical industry.

Cell-Based Expression Systems	Cell-Free Expression Systems
Bacteria expression system	Escherichia coli extract (ECE)
Yeast expression system	Rabbit reticulocyte lysate (RRL)
Insect expression system	Saccharomyces cerevisiae extract (SCE)
Algal expression system	Insect cell extract (ICE)
Plant-based expression system	Wheat germ extract (WGE)
Mammalian expression system	Tobacco BY-2 cell extract (BYE)
Transient protein expression	CHO, K562, and heLa extracts
Stable protein expression	Leishmania tarentolae extract (LTE)
Cell-based membrane protein expression	Cell-free membrane protein expression
High-cell-density protein expression system	Chemical protein synthesis

Available Services

Creative BioMart offers a full package of services for custom protein production to meet any research need. Our scientific team is dedicated to helping our customers choose and optimize the most appropriate approach to generate proteins with high purity and high yields for each project. Our services include, but are not limited to the following:

Phase Ⅰ Services	Phase Ⅱ Services	Add-On Services
Gene synthesis and cloning	High yield protein production	Protein labeling and conjugation	Tag removal
Cell line development	Fermentation	Protein refolding	Protein engineering
Protein expression	Endotoxin removal & testing	Post-translational modification	Functional assays
Protein purification	QC & delivery	Assay development	Antibody development

Start from choosing one or more services above. If you can't find what you are looking for in the options above, please fill out the online inquiry form and let us know more details.

Delivery Specifications

• Protein production is under strict quality control. QC reports will be delivered along with your proteins.
• Proteins will be delivered in the format you need.
• For long-term storage, proteins should be stored at -20°C or colder and away from bright light after receiving.

Custom Project Workflow

Why Choose Us

• Extensive experience working with various recombinant expression systems and protein classes.
• Up-to-date knowledge in protein technologies and state-of-the-art techniques.
• Guaranteed quality, consistency, reliability, and reproductivity.
• Scalable platform and customized services for specific needs.
• Fast turnaround time and competitive price.

Creative BioMart has a strong team of chemists, biologists, and pharmacologists working on your project to save your time and effort. Your protein may already be available in our product catalog. If you require any further information, please feel free to contact us. We look forward to working together to meet your goals.

References

• Bhoir S, et al. High yield bacterial expression, purification and characterisation of bioactive Human Tousled-like Kinase 1B involved in cancer. Scientific reports, 2018, 8(1): 1-9.
• Hunt I. From gene to protein: a review of new and enabling technologies for multi-parallel protein expression. Protein expression and purification, 2005, 40(1): 1-22.