Labeled Ligand Binding Assays
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INQUIRYCreative BioMart has developed a large collection of labeled ligand binding assays that allow for high-quality and efficient kinase screening & profiling to accelerate kinase research and drug discovery. With years of experience in kinase biology and drug development, we can help our customers overcome challenges and accelerate their journey to success.
Background
Kinases comprise one of the most abundant gene families in humans and belong to one of the largest families of evolutionary related proteins. The human kinome comprises 518 protein kinases. Kinases can phosphorylate the serine, threonine, tyrosine, or histidine residues in proteins and modify their functional activity in cells. Over the last couple of decades, kinases have proven to be one the most important and effective therapeutic targets for various diseases.
Ligand binding assays are powerful tools to quantitatively measure the binding of small molecules to kinases. Labeled ligand binding assays involving fluorescence, bioluminescence and radiolabeling are valuable in determining the binding affinity, kinetics and thermodynamics, ligand efficiency, and molecular conformations of targets. The development, implementation, and validation of reliable labeled ligand binding assays to characterize kinase binding properties is essential for successful drug discovery.
Figure 1. Schematic illustration of FP principle in relation to C1) the IMAP technology, C2) use of cationic polyamino acids, C3) biotinylation and addition of streptavidin. (Zinkle A & Mohammadi M., 2018)
Service Options
Creative BioMart offers comprehensive options for labeled ligand binding assays to meet the needs of different customers. Our scientific team will help our customers select suitable assays and develop an optimal strategic plan. If you have any additional requirements, please fill out the online inquiry form and let us know more details.
Fluorescent Ligand Binding Assays
Fluorescent labeled ligand binding assays have a broad spectrum of wavelengths thus can apply multiple colors. These assays can be applied to detect its binding to a target. Creative BioMart provides multiple techniques for the measurement of fluorescence intensity, including but not limited to fluorescence polarization, time-resolved fluorescence, fluorescence anisotropy, fluorescence correlation spectroscopy, and fluorescence resonance energy transfer. We have also developed kinase microarray-based fluorescent assays.
Radiometric Ligand Binding Assays
A radioactively labeled ligand can be applied to determine ligand affinity and binding sites. Creative BioMart provides different types of radioligand binding assays including saturation assay, competitive assay, and kinetic assay to provide useful information on the selectivity of kinase drugs and inhibitors against the human kinome.
Bioluminescent Ligand Binding Assays
Bioluminescence has been widely used because of its high sensitivity, low background, and wide linear range. Bioluminescent binding assays are non-radioactive assays for the quantitative measurements of ligand-receptor interactions. Creative BioMart has developed a novel nanoluciferase-based bioluminescent binding assays with the advantages of high sensitivity, stability, and reproducibility.
Creative BioMart is an advanced biotech company within the field of kinase/phosphatase biology. We offer our expertise in establishing assays for kinase screening & profiling. We have state-of-the-art facilities and technology to provide efficient services with guaranteed turnaround times. Any data or details you provide will be kept strictly confidential. If you have any questions, please do not hesitate to contact us.
References
- Lea W A & Simeonov A. Fluorescence polarization assays in small molecule screening. Expert opinion on drug discovery, 2011, 6(1): 17-32.
- Wang Y & Ma H. Protein kinase profiling assays: a technology review. Drug Discovery Today: Technologies, 2015, 18: 1-8.