Protein Engineering for Phosphorylation Research
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INQUIRYCreative BioMart offers a comprehensive portfolio of protein engineering services to accelerate kinase/phosphatase biology research and pharmaceutical development. We have a long and successful history of providing protein engineering services to help our customers accelerate their projects and overcome a wide range of challenges.
Background
Protein engineering is a powerful biotechnological tool for the development of useful or valuable proteins to meet the needs of different industrial applications. Two common strategies of protein engineering include directed evolution for proteins with limited structure information and unclear mechanism, and rational design for proteins with well-known structure and mechanism. Protein engineering is a powerful tool to tune protein function for the desired application.
In recent years, advances in protein engineering have led to new approaches for manipulating the activity, localization, and specificity of kinases and phosphatases. Numerous strategies for engineering kinase/phosphatase regulation have been developed to create kinases or phosphatases with desired properties used for unraveling the fundamental principles of intracellular signal transduction, facilitating biological screening, and designing therapeutic allosteric regulators.
Figure 1. Strategies for semisynthesis of protein phosphorylation. (Chen Z & Cole P A., 2015)
Available Services
Creative BioMart offers a full package of services to provide our customers with maximum flexibility to meet their needs in the life sciences and pharmaceutical industry. Our scientific team is dedicated to helping our customers choose and optimize the most appropriate strategy for each project. Our services include, but are not limited to the following:
| Directed Evolution | Rational Design | Add-On Services |
| Random mutagenesis | Protein structure determination | Protein production and purification |
| Error prone PCR | Structure-based design | Phosphorylation and dephosphorylation |
| Sequence saturation mutagenesis | Sequence-based design | Protein analysis |
| DNA shuffling | De novo design | Engineering new catalytic activities in enzymes |
| Mutant library construction | Site-directed mutagenesis | Unnatural amino acid mutagenesis-based enzyme engineering |
| Staggered extension | Computer-aided design | Artificial enzyme construction |
| Incremental truncation | Semi-rational design | Antibody engineering |
| Selection and screening | Bioinformatics | Assay validation |
Start from choosing one or more services above. If you can't find what you are looking for in the options above, please fill out the online inquiry form and let us know more details.
Custom Project Workflow
Why Choose Us
- Strong in-depth knowledge and extensive hands-on experience in protein/enzyme engineering.
- State-of-the-art techniques, scalable platform and customized services for specific needs.
- Guaranteed quality, reliability, and reproductivity.
- Fast turnaround time and competitive price.
Creative BioMart has a strong team of chemists, biologists, and pharmacologists working on your project to save your time and effort. Your protein may already be available in our product catalog. If you require any further information, please feel free to contact us. We look forward to working together to meet your goals.
References
- Chen Z & Cole P A. Synthetic approaches to protein phosphorylation. Current opinion in chemical biology, 2015, 28: 115-122.
- McCormick J W, et al. Strategies for engineering and rewiring kinase regulation. Trends in biochemical sciences, 2020, 45(3): 259-271.