FES (NM_002005) Human Untagged Clone
Product Information
| Product Name | FES (NM_002005) Human Untagged Clone |
| Cat.No | CDL25136 |
| Description | FES (untagged)-Kinase deficient mutant (K590M) of Human feline sarcoma oncogene (FES), transcript variant 1 |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Symbol | FES |
| Synonyms | FPS |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | Fully Sequenced ORF (showhide) >OriGene ORF within SC323596 sequence for NM_002005 edited (data generated by NextGen Sequencing) ATGGGCTTCTCTTCCGAGCTGTGCAGCCCCCAGGGCCACGGGGTCCTGCAGCAAATGCAG GAGGCCGAGCTTCGTCTACTGGAGGGCATGAGAAAGTGGATGGCCCAGCGGGTCAAGAGT GACAGGGAGTATGCAGGACTGCTTCACCACATGTCCCTGCAGGACAGTGGGGGCCAGAGC CGGGCCATCAGCCCTGACAGCCCCATCAGTCAGTCCTGGGCTGAGATCACCAGCCAAACT GAGGGCCTGAGCCGCTTGCTGCGGCAGCACGCAGAGGATCTGAACTCAGGGCCCCTGAGC AAGCTGAGCCTGCTCATCCGGGAACGGCAGCAGCTTCGCAAGACCTACAGCGAGCAGTGG CAGCAGCTGCAGCAGGAGCTCACCAAGACCCACAGCCAGGACATTGAGAAGCTGAAGAGC CAGTACCGAGCTCTGGCACGGGACAGTGCCCAAGCCAAGCGCAAGTACCAGGAGGCCAGC AAAGACAAGGACCGTGACAAGGCTAAGGACAAGTATGTGCGCAGCCTGTGGAAGCTCTTT GCTCACCACAACCGCTATGTGCTGGGCGTGCGGGCTGCGCAGCTACACCACCAGCACCAC CACCAGCTCCTGCTGCCCGGCCTGCTGCGGTCACTGCAGGACCTGCACGAGGAGATGGCT TGCATCCTGAAGGAGATCCTGCAGGAATACCTGGAGATTAGCAGCCTGGTGCAGGATGAG GTGGTGGCCATTCACCGGGAGATGGCTGCAGCTGCTGCCCGCATCCAGCCTGAGGCTGAG TACCAAGGCTTCCTGCGACAGTATGGGTCCGCACCTGACGTCCCACCCTGTGTCACGTTC GATGAGTCACTGCTTGAGGAGGGTGAACCGCTGGAGCCTGGGGAGCTCCAGCTGAACGAG CTGACTGTGGAGAGCGTGCAGCACACGCTGACCTCAGTGACAGATGAGCTGGCTGTGGCC ACCGAGATGGTGTTCAGGCGGCAGGAGATGGTTACGCAGCTGCAACAGGAGCTCCGGAAT GAAGAGGAGAACACCCACCCCCGGGAGCGGGTGCAGCTGCTGGGCAAGAGGCAAGTGCTG CAAGAAGCACTGCAGGGGCTGCAGGTAGCGCTGTGCAGCCAGGCCAAGCTGCAGGCCCAG CAGGAGTTGCTGCAGACCAAGCTGGAGCACCTGGGCCCCGGCGAGCCCCCGCCTGTGCTG CTCCTGCAGGATGACCGCCACTCCACGTCGTCCTCGGAGCAGGAGCGAGAGGGGGGAAGG ACACCCACGCTGGAGATCCTTAAGAGCCACATCTCAGGAATCTTCCGCCCCAAGTTCTCG CTCCCTCCACCGCTGCAGCTCATTCCGGAGGTGCAGAAGCCCCTGCATGAGCAGCTGTGG TACCACGGGGCCATCCCGAGGGCAGAGGTGGCTGAGCTGCTGGTGCACTCTGGGGACTTC CTGGTGCGGGAGAGCCAGGGCAAGCAGGAGTACGTGCTGTCGGTGCTGTGGGATGGTCTG CCCCGGCACTTCATCATCCAGTCCTTGGATAACCTGTACCGACTGGAAGGGGAAGGCTTT CCTAGCATTCCTTTGCTCATCGACCACCTACTGAGCACCCAGCAGCCCCTCACCAAGAAG AGTGGTGTTGTCCTGCACAGGGCTGTGCCCAAGGACAAGTGGGTGCTGAACCATGAGGAC CTGGTGTTGGGTGAGCAGATTGGACGGGGGAACTTTGGCGAAGTGTTCAGCGGACGCCTG CGAGCCGACAACACCCTGGTGGCGGTGAAGTCTTGTCGAGAGACGCTCCCACCTGACCTC AAGGCCAAGTTTCTACAGGAAGCGAGGATCCTGAAGCAGTACAGCCACCCCAACATCGTG CGTCTCATTGGTGTCTGCACCCAGAAGCAGCCCATCTACATCGTCATGGAGCTTGTGCAG GGGGGCGACTTCCTGACCTTCCTCCGCACGGAGGGGGCCCGCCTGCGGGTGAAGACTCTG CTGCAGATGGTGGGGGATGCAGCTGCTGGCATGGAGTACCTGGAGAGCAAGTGCTGCATC CACCGGGACCTGGCTGCTCGGAACTGCCTGGTGACAGAGAAGAATGTCCTGAAGATCAGT GACTTTGGGATGTCCCGAGAGGAAGCCGATGGGGTCTATGCAGCCTCAGGGGGCCTCAGA CAAGTCCCCGTGAAGTGGACCGCACCTGAGGCCCTTAACTACGGCCGCTACTCCTCCGAA AGCGACGTGTGGAGCTTTGGCATCTTGCTCTGGGAGACCTTCAGCCTGGGGGCCTCCCCC TATCCCAACCTCAGCAATCAGCAGACACGGGAGTTTGTGGAGAAGGGGGGCCGTCTGCCC TGCCCAGAGCTGTGTCCTGATGCCGTGTTCAGGCTCATGGAGCAGTGCTGGGCCTATGAG CCTGGGCAGCGGCCCAGCTTCAGCACCATCTACCAGGAGCTGCAGAGCATCCGAAAGCGG CATCGGTGA Clone variation with respect to NM_002005.3 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for mutant NM_002005 unedited ACCGCACGTTGAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCAGGAGCAGCTGCC CGTGCGGGTACCTCTAGCCCCGGGGCCTGGAGGAGCGGTGGGAGCTGGGGGCGCGGCAGGCAGGGGCAGA GCAGGCGTTCCGAGGGCCAGAGACCCACCCAGAACAGCACTATGGGCTTCTCTTCCGAGCTGTGCAGCCC CCAGGGCCACGGGGTCCTGCAGCAAATGCAGGAGGCCGAGCTTCGTCTACTGGAGGGCATGAGAAAAGTG GATGGCCCCAGCGGGTCAAGAGTGACAGGGAGTATGCAGGACTGCTTTAACCACATGTCCCTGCAGAAAT GGGGGGCCAGAGCCGGCCCTTCACCCTTGACAGCCCCATCATTCATTCCGGGGTTGAGATACCCAGCCAA CTTGAGGCCCTGACCCGCTTTGCTCCGGCAGCACGCAAAGGATCTGACTTCAGGGCCCTTGACCAAGCTG ACCCTGCTATTCGGGAACGGGCAACGCGCTTCCGCAGAAACCTACAGAGACATGTGACACCACTCTGAAA CGAGATCTACCCGAGACCCCACGCCGGGACTTGGAAAGCTAAAGACCCTGTCCAGATCTTGGGCCGGGGA CAGGCCCCAGCCACGCCAGTCCAGAGGCCACAAACAGGCCCGTAACGGGCCAGGAAATTGTGCGCCTCGG AAATCTTTTGCTCACACACTTTTTCTGGGTTGGGTTGGCAATCACAAAGCCCACATTTTGGTGGCGCTGG GGGGTCATGACACCAGAAGAGATGCTACTCTGACTCAGGTACTCCCAGTGCACTAGAACCCCTTGAGAGG AAGCTGTCGCGTAGAAACTTCAGGACGATCTT Kinase domain sequence (showhide) >SC323596 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutationATGGKACAGATGGMGGGGACTTTGGCGAGTGTTCAGCGGACGCCTGCGAGCCGACAACACCCTGGTGGCG GTGATGTCTTGTCGAGAGACGCTCCCACCTGACCTCAAGGCCAAGTTTCTACAGGAAGCGAGGATCCTGA AGCAGTACAGCCACCCCAACATCGTGCGTCTCATTGGTGTCTGCACCCAGAAGCAGCCCATCTACATCGT CATGGAGCTTGTGCAGGGGGGCGACTTCCTGACCTTCCTCCGCAC |
| Restriction Sites1 | Please inquire |
| ACCN | NM_002005 |
| Insert Size | 3000 bp |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| RefSeq | NM_002005.2, NP_001996.1 |
| RefSeq Size | 2762 bp |
| RefSeq ORF | 2469 bp |
| Locus ID | 2242 |
| UniProt ID | P07332 |
| Cytogenetics | 15q26.1 |
| Domains | pkinase, SH2, FCH, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Axon guidance |
| Gene Summary | This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia but it is also involved in normal hematopoiesis as well as growth factor and cytokine receptor signaling. Alternative splicing results in multiple variants encoding different isoforms.[provided by RefSeq, Jan 2009]Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |