HIPK3 (NM_005734) Human Untagged Clone
Product Information
| Product Name | HIPK3 (NM_005734) Human Untagged Clone |
| Cat.No | CDL25141 |
| Description | HIPK3 (untagged)-Kinase deficient mutant (K226M) of Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 1 |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Symbol | HIPK3 |
| Synonyms | DYRK6; FIST3; PKY; YAK1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_005734, the custom clone sequence may differ by one or more nucleotides ATGGCCTCACAAGTCTTGGTCTACCCACCATATGTTTATCAAACTCAGTCAAGTGCCTTTTGTAGTGTGA AGAAACTCAAAGTAGAGCCAAGCAGTTGTGTATTCCAGGAAAGAAACTATCCACGGACCTATGTGAATGG TAGAAACTTTGGAAATTCTCATCCTCCCACTAAGGGTAGTGCTTTTCAGACAAAGATACCATTTAATAGA CCTCGAGGACACAACTTTTCATTGCAGACAAGTGCTGTTGTTTTGAAAAACACTGCAGGTGCTACAAAGG TCATAGCAGCTCAGGCACAGCAAGCTCACGTGCAGGCACCTCAGATTGGGGCGTGGCGAAACAGATTGCA TTTCCTAGAAGGCCCCCAGCGATGTGGATTGAAGCGCAAGAGTGAGGAGTTGGATAATCATAGCAGCGCA ATGCAGATTGTCGATGAATTGTCCATACTTCCTGCAATGTTGCAAACCAACATGGGAAATCCAGTGACAG TTGTGACAGCTACCACAGGATCAAAACAGAATTGTACCACTGGAGAAGGTGACTATCAGTTAGTACAGCA TGAAGTCTTATGCTCCATGAAAAATACTTACGAAGTCCTTGATTTTCTTGGTCGAGGCACGTTTGGCCAG GTAGTTAAATGCTGGAAAAGAGGGACAAATGAAATTGTAGCAATCAAAATTTTGAAGAATCATCCTTCTT ATGCCCGTCAAGGTCAAATAGAAGTGAGCATATTAGCAAGGCTCAGTACTGAAAATGCTGATGAATATAA CTTTGTACGAGCTTATGAATGCTTTCAGCACCGTAACCATACTTGTTTAGTCTTTGAGATGCTGGAACAA AACTTGTATGACTTTCTGAAACAAAATAAATTTAGTCCCCTGCCACTAAAAGTGATTCGGCCCATTCTTC AACAAGTGGCCACTGCACTGAAAAAATTGAAAAGTCTTGGTTTAATTCATGCTGATCTCAAGCCAGAGAA TATTATGTTGGTGGATCCTGTTCGGCAGCCTTACAGGGTTAAAGTAATAGACTTTGGGTCGGCCAGTCAT GTATCAAAGACTGTTTGTTCAACATATCTACAATCTCGGTACTACAGAGCTCCAGAGATTATATTGGGGT TGCCATTTTGTGAAGCCATAGACATGTGGTCATTGGGATGTGTGATTGCAGAATTATTTCTTGGATGGCC GCTCTACCCAGGAGCCTTGGAGTATGATCAGATTCGATACATTTCTCAGACTCAAGGTTTGCCAGGAGAA CAGTTGTTAAATGTGGGTACTAAATCCACAAGATTTTTTTGCAAAGAAACAGATATGTCTCATTCTGGTT GGAGATTAAAGACATTGGAAGAGCATGAGGCAGAGACAGGAATGAAGTCTAAAGAAGCCAGAAAATACAT TTTCAACAGTCTGGATGATGTAGCGCATGTGAACACAGTGATGGATTTGGAAGGAAGTGATCTTTTGGCT GAGAAAGCTGATAGAAGAGAATTTGTTAGTCTGTTGAAGAAAATGTTGCTGATTGATGCAGATTTAAGAA TTACTCCAGCTGAGACCCTGAACCATCCTTTTGTTAATATGAAACATCTTCTAGATTTCCCTCATAGCAA CCATGTAAAGTCCTGTTTTCATATTATGGATATTTGTAAGTCCCACCTAAATTCATGTGACACAAATAAT CACAACAAAACTTCACTTTTAAGACCAGTTGCTTCAAGCAGTACTGCTACACTGACTGCAAATTTTACTA AAATCGGAACATTAAGAAGTCAGGCATTGACCACATCTGCTCATTCAGTTGTGCACCATGGAATACCTCT GCAGGCAGGAACTGCTCAGTTTGGTTGTGGTGATGCTTTTCAGCAGACATTGATTATCTGTCCCCCAGCT ATTCAAGGTATTCCTGCAACACATGGTAAACCCACCAGTTATTCAATAAGGGTAGATAATACAGTTCCAC TTGTAACTCAGGCCCCAGCTGTGCAGCCACTACAGATCCGACCAGGAGTTCTTTCTCAGACGTGGTCTGG TAGAACACAGCAGATGCTGGTGCCTGCCTGGCAACAGGTGACACCCCTGGCTCCTGCTACTACTACACTA ACTTCTGAGAGTGTGGCTGGTTCACACAGGCTTGGAGACTGGGGGAAGATGATTTCATGCAGCAATCATT ATAACTCAGTGATGCCGCAGCCTCTTCTGACCAATCAGATAACTTTATCTGCCCCTCAGCCAGTTAGTGT GGGGATTGCACATGTTGTCTGGCCTCAGCCTGCCACTACCAAGAAAAATAAACAGTGCCAGAACAGAGGT ATTTTGGTAAAACTAATGGAATGGGAGCCAGGAAGAGAGGAAATAAATGCTTTCAGTTGGAGTAATTCAT TACAGAATACCAATATCCCACATTCAGCATTTATTTCTCCAAAGATAATTAATGGGAAAGATGTCGAGGA AGTAAGTTGTATAGAAACACAGGACAATCAGAACTCAGAAGGAGAGGCAAGAAATTGCTGTGAAACATCT ATCAGACAGGACTCTGATTCATCAGTTTCAGACAAACAGCGGCAAACCATCATTATTGCCGACTCCCCGA GTCCTGCAGTGAGTGTCATCACTATCAGCAGTGACACTGATGAGGAAGAGACTTCCCAGAGACATTCACT CAGAGAATGTAAAGGTAGTCTAGATTGTGAAGCTTGCCAGAGCACTTTGAATATTGATCGGATGTGTTCA TTAAGTAGTCCTGATAGTACTCTGAGTACCAGCTCCTCAGGGCAGTCCAGCCCATCCCCCTGCAAGAGAC CGAATAGTATGTCAGATGAAGAGCAAGAAAGTAGTTGTGATACGGTGGATGGCTCTCCGACATCTGACTC TTCCGGGCATGACAGTCCATTTGCAGAGAGCACTTTTGTGGAGGACACTCATGAAAACACAGAATTGGTA TCCTCTGCTGACACAGAAACCAAGCCAGCTGTCTGTTCTGTTGTGGTGCCACCAGTGGAACTAGAAAATG GCTTAAATGCCGATGAGCATATGGCAAACACAGATTCTATATGCCAGCCATTAATAAAAGGACGATCTGC CCCTGGAAGATTAAACCAGCCTTCTGCAGTGGGTACTCGTCAGCAAAAATTGACATCAGCATTCCAGCAG CAGCATTTGAACTTCAGTCAGGTTCAGCACTTTGGATCTGGGCATCAAGAGTGGAATGGAAACTTTGGGC ACAGAAGACAGCAAGCTTATATTCCTACTAGTGTTACCAGTAATCCATTCACTCTTTCTCATGGAAGTCC CAATCACACAGCAGTGCATGCCCACCTGGCTGGAAATACACACCTCGGAGGACAGCCTACTCTACTTCCA TACCCATCATCAGCCACCCTCAGTAGTGCTGCACCAGTGGCCCACCTGTTAGCCTCTCCGTGTACCTCAA GACCTATGTTACAGCATCCAACTTATAATATCTCCCATCCCAGTGGCATAGTTCACCAAGTCCCAGTGGG CTTAAATCCCCGTCTGTTACCATCCCCAACCATTCATCAGACTCAGTACAAACCAATCTTCCCACCACAT TCTTACATTGCAGCATCACCTGCATATACTGGATTTCCACTGAGTCCAACAAAACTCAGCCAGTATCCAT ATATGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for mutant NM_005734 unedited ACCCGCCCGTTGCAGCAATGGGCGGTAGGCGTGTACAGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAG TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAAGATGAGG GAGACGGGCCCGGCGCTTAGCAGCCAGAGCAGCAGCAGCAGCAGCAGCGGTCGGGGGAGGGTGTTTCGCC GTTTCCTCTCAGCCGCCAGGACAAGATGGCAGCGGCCGCGGAGAGGGGCTGAGCCCGGGCTGGGTGGTGC CGCCTGCTGAAGCGCCTGGCTCCCGGTCCCCGGCACGGCCCTGCGCCCCCCCCCCGGACATGCTCAGGGC TGCGGGCCGCCCGAAAAAGGAGAGAGCGCGGGCCTCTAGGAAGGTATGGCCTCCCAAGTCTGGGTCTAAC CCCCCATATGTTTATTCAAATCAGTCCAATGGCCTTTGGTATGGGGAGAAAACTCAAAGTAAAAGCCAAC CAGTTGGGTTTTCCAGGAAAAAAACTATCCCCCGGACCTTTGGGATGGGGAAAAACTTTGGAAAATTCCC ATCTTCCCCCAAGGGGTAGGCCTTTTCAAAACAAAAATCCCTTTTAATAGACCCTCGGGGACCAACTTTT TCATTTCCAAAAAAAGGCTTTTGTTTTGAAAAAACCCTGGAGGGGCCCCCAAAGGGTAAAAGCCTCTAGG GCACACAGTTCCCTGTCGCGGGCCCCTAAAAGGGGGGGTGTGGAAAAAAAAATGTGTTTTTCATAAGAGC CCCCCCCACATGTGTGTTTTGCGCCCCAGAGGAGTGAGGTGTGTGATAATCTATACGCCCAGTCGCGATG TGTGTCAAGATATTCGCACTCTCCTCCGCGTGTGTGACACACAGTGAGAATCCGCTGCGTGTTGACACAC CACAGCTCACAATTATTCCCCGGAGGAGAGGTATCTATATTATTCACGCGGAGTATTTCTCGCCGCAAAA TATACATGACTAG Kinase domain sequence (showhide) >SC323551 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutationARTCTTGWTTTCTTGGTCGAGGCACGTTTGGCCAGGTAGTTAAATGCTGGAAAAGAGGGACAAATGAAAT TGTAGCAATCATGATTTTGAAGAATCATCCTTCTTATGCCCGTCAAGGTCAAATAGAAGTGAGCATATTA GCAAGGCTCAGTACTGAAAATGCTGATGAATATAACTTTGTACGAGCTTATGAATGCTTTCAGCACCGTA ACCATACTTGTTTAGTCTTTGAGATGCTGGAACAAAACTTGTATG |
| Restriction Sites1 | Please inquire |
| ACCN | NM_005734 |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| RefSeq | NM_005734.2, NP_005725.2 |
| RefSeq Size | 3657 bp |
| RefSeq ORF | 3648 bp |
| Locus ID | 10114 |
| UniProt ID | Q9H422 |
| Cytogenetics | 11p13 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase, Transcription Factors |
| Gene Summary | Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).[UniProtKB/Swiss-Prot Function]Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |