Human CKMT1A Knockout HEK293T Cell Lysate

Storage Condition	Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
Shipping Condition	Shipped on dry ice.

Parental Cell Line	HEK293T
Passage Number	<20
Mutation Description	CRISPR/Cas9 system is used to generate knockout cell lines. Mutation: 1 bp insertion in exon4.
Cell Type	Epithelial Cells
Mycoplasma Free	Yes
Knockout Validation	Sanger Sequencing
Lysis Buffer	RIPA buffer added with a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
Description	As WB control, the lyophilizate is recommended to be resuspend in 50 µL of LDS Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, a final concentration of 0.1M DTT is recommended.

Target Function	Creatine kinase MT is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. Overexpression of ubiquitous mitochondrial creatine kinase is involved in many malignant cancers with poor prognosis.
Cellular Localization	Cell membrane and mitochondrial.
Target Sequence Similarities	Belongs to the creatine kinase isoenzyme family.