Human CSNK1D Knockout HeLa Cell Lysate

Storage Condition	Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
Shipping Condition	Shipped on dry ice.

Parental Cell Line	HeLa
Passage Number	<20
Mutation Description	CRISPR/Cas9 system is used to generate knockout cell lines. Mutation: 11 bp insertion in exon 1 and 1 bp deletion in exon 1.
Cell Type	Epithelial Cells
Mycoplasma Free	Yes
Knockout Validation	Sanger Sequencing, Western Blot (WB)
Lysis Buffer	RIPA buffer added with a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
Disease	Adenocarcinoma
Gender	Female
Applications	Western blot. Predicted molecular weight: 47 kDa.
Description	As WB control, the lyophilizate is recommended to be resuspend in 50 µL of LDS Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, a final concentration of 0.1M DTT is recommended.

Cellular Localization	Cytoplasm and nucleus.
Tissue Specificity	Expressed in all tissues examined, including brain, heart, lung, liver, kidney, skeletal muscle, pancreas, and placenta.
Target Sequence Similarities	Belongs to the CK1 Ser/Thr protein kinase family, Casein kinase I subfamily.
Post-Translational Modifications	Autophosphorylated on serine and threonine residues.