Human PIK3R3 Knockout THP-1 Cell Line

Storage Condition	Upon arrival, the vial should be stored in liquid nitrogen vapor phase. Storage buffer: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether
Shipping Condition	Shipped on dry ice.

Parental Cell Line	THP-1
Passage Number	<20
Viability	~80%
Adherent / Suspension	Suspension
Cell Type	Monocytic Cells
Mycoplasma Free	Yes
Knockout Validation	Sanger Sequencing
Cell Medium	Culture medium: RPMI + 10% FBS. + 0.05 mM β-mercaptoethanol Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Initial Handling Guidelines	1. Thaw the vial in 37ºC water bath approximately 1-2 minutes. 2. Transfer the cell suspension to a 15 ml/50 ml conical sterile polypropylene centrifuge tube containing pre-warmed culture medium. Wash vial with an additional culture medium to collect remaining cells, and centrifuge at 201 × g (rcf) for 5 minutes at room temperature. 3. Resuspend the cell pellet in 5 ml pre-warmed culture medium and count cell number. Seed cells in an appropriate cell culture flask at a density of 2×10⁴ cells/cm². This should allow for confluency within 48 hours. 4. Incubate the culture at 37ºC incubator with 5% CO₂. Cultures should be monitored daily.
Subculture Guidelines	Cells should be passaged when they have achieved 80-90% confluence. A partial media change 24 hours prior to subculture may be helpful to encourage growth.
Disease	Acute monocytic leukemia
Description	Human wild-type THP-1 cell line as the recommended control.

Tissue Specificity	Highest expression level in brain and testis. Lower expression level in heart, lung, kidney, adipose, and skeletal muscle.
Target Sequence Similarities	Belongs to the PI3K p85 subunit family.