MEK1 (MAP2K1) (NM_002755) Human Untagged Clone
Product Information
| Product Name | MEK1 (MAP2K1) (NM_002755) Human Untagged Clone |
| Cat.No | CDL25144 |
| Description | MAP2K1 (untagged)-Kinase deficient mutant (K97M) of Human mitogen-activated protein kinase kinase 1 (MAP2K1) |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Symbol | MAP2K1 |
| Synonyms | CFC3; MAPKK1; MEK1; MEL; MKK1; PRKMK1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | Fully Sequenced ORF (showhide) >OriGene ORF within SC323696 sequence for NM_002755 edited (data generated by NextGen Sequencing) ATGCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGACGGCTCTGCAGTT AACGGGACCAGCTCTGCGGAGACCAACTTGGAGGCCTTGCAGAAGAAGCTGGAGGAGCTA GAGCTTGATGAGCAGCAGCGAAAGCGCCTTGAGGCCTTTCTTACCCAGAAGCAGAAGGTG GGAGAACTGAAGGATGACGACTTTGAGAAGATCAGTGAGCTGGGGGCTGGCAATGGCGGT GTGGTGTTCAAGGTCTCCCACAAGTCTTCTGGCCTGGTCATGGCCAGAATGCTAATTCAT CTGGAGATCAAACCCGCAATCCGGAACCAGATCATAAGGGAGCTGCAGGTTCTGCATGAG TGCAACTCTCCGTACATCGTGGGCTTCTATGGTGCGTTCTACAGCGATGGCGAGATCAGT ATCTGCATGGAGCACATGGATGGAGGTTCTCTGGATCAAGTCCTGAAGAAAGCTGGAAGA ATTCCTGAACAAATTTTAGGAAAAGTTAGCATTGCTGTAATAAAAGGCCTGACATATCTG AGGGAGAAGCACAAGATCATGCACAGAGATGTCAAGCCCTCCAACATCCTAGTCAACTCC CGTGGGGAGATCAAGCTCTGTGACTTTGGGGTCAGCGGGCAGCTCATCGACTCCATGGCC AACTCCTTCGTGGGCACAAGGTCCTACATGTCGCCAGAAAGACTCCAGGGGACTCATTAC TCTGTGCAGTCAGACATCTGGAGCATGGGACTGTCTCTGGTAGAGATGGCGGTTGGGAGG TATCCCATCCCTCCTCCAGATGCCAAGGAGCTGGAGCTGATGTTTGGGTGCCAGGTGGAA GGAGATGCGGCTGAGACCCCACCCAGGCCAAGGACCCCCGGGAGGCCCCTTAGCTCATAC GGAATGGACAGCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTACATAGTCAACGAG CCTCCTCCAAAACTGCCCAGTGGAGTGTTCAGTCTGGAATTTCAAGATTTTGTGAATAAA TGCTTAATAAAAAACCCCGCAGAGAGAGCAGATTTGAAGCAACTCATGGTTCATGCTTTT ATCAAGAGATCTGATGCTGAGGAAGTGGATTTTGCAGGTTGGCTCTGCTCCACCATCGGC CTTAACCAGCCCAGCACACCAACCCATGCTGCTGGCGTCTAA Clone variation with respect to NM_002755.3265 c=>t;290 a=>t 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for mutant NM_002755 unedited GCCGCCGTTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTGAGAGAGAGA GAGGAAGGGAATCCCGGGCTGCCGAACCGCACGTTCAGCCCGCTCCGCTCCTGCAGGGCAGCCTTTCGGC TCTCTGCGCGCGAAGCCGAGTCCCGGGCGGGTGGGGCGGGGGTCCACTGAGACCGCTACCGGCCCCTCGG CGCTGACGGGACCGCGCGGGGGCGCACCCGCTGAAGGCAGCCCCGGGGCCCCGCGGGCCCGGACTTTGGT CCTGCGCAGCGGGGCGCGGGGGCAGCGCAGCGGGGAGGAGCCAAGAAGGTGCTGCCCTTCCCCCGGAATT GGGAGCCCCGTTACCCGGGTCCAAAAGGCCCAAAAGAAGCCCAACCCCCTTCCACCTGACCCCGGCCCCC GCCGGCTTGGCAGTTAACGGGACCAGCTTTCGGGAACCAAACTGGGAGGCCTTGCAAAAAAACCTGGAGG GCCTAACTTTGGTGACCACACGAAAAGCCCTTGGGCCTTTTTTACCCAACCAAGGGGGAAAACTGAGGAA CACATTTGAAATATCGTGCCGGGCGCGGATGGGGGGTGTGTTAGGTCCCAATTCTGGCCGTGTGGGCCAT GTGCATTCTGGATATACCCATCTGGACCATACAGAGACCGCGCTCTGATCGGACACTCCACTAGGGTCTA GGGGGGCT Kinase domain sequence (showhide) >SC323696 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutationCYCTGMGCAATGGGCGKAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTGAGAGAGAGAGAGGAAG GGAATCCCGGGCTGCCGAACCGCACGTTCAGCCCGCTCCGCTCCTGCAGGGCAGCCTTTCGGCTCTCTGC GCGCGAAGCCGAGTCCCGGGCGGGTGGGGCGGGGGTCCACTGAGA |
| Restriction Sites1 | Please inquire |
| ACCN | NM_002755 |
| Insert Size | 2550 bp |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| RefSeq | NM_002755.2, NP_002746.1 |
| RefSeq Size | 2222 bp |
| RefSeq ORF | 1182 bp |
| Locus ID | 5604 |
| UniProt ID | Q02750 |
| Cytogenetics | 15q22.31 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Oocyte meiosis, Pancreatic cancer, Pathways in cancer, Prion diseases, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway |
| Gene Summary | The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008] |