TAOK1 (NM_020791) Human Tagged ORF Clone
Product Information
| Product Name | TAOK1 (NM_020791) Human Tagged ORF Clone |
| Cat.No | CDEP1007 |
| Description | TAOK1 (Myc-DDK-tagged)-Human TAO kinase 1 (TAOK1), transcript variant 1 |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | TAOK1 |
| Synonyms | hKFC-B; hTAOK1; KFC-B; MAP3K16; MARKK; PSK-2; PSK2; TAO1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Sequence Data | ORF Nucleotide Sequence (showhide) >RC219490 representing NM_020791 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCATCAACTAACAGAGCAGGCAGCCTGAAGGACCCTGAAATTGCAGAGCTCTTCTTCAAAGAAGATC CAGAGAAGCTCTTCACAGATCTCAGAGAAATTGGCCATGGAAGCTTTGGAGCAGTGTATTTTGCACGAGA TGTGCGTACCAATGAAGTGGTGGCCATCAAGAAAATGTCTTATAGTGGAAAGCAGTCTACTGAGAAATGG CAGGATATTATTAAGGAAGTCAAGTTTCTACAAAGAATAAAACATCCCAACAGTATAGAATACAAAGGCT GTTATTTACGTGAACACACAGCATGGCTTGTAATGGAATATTGTTTAGGATCTGCTTCGGATTTACTAGA AGTTCACAAAAAGCCATTACAAGAAGTGGAAATAGCAGCAATTACACATGGTGCTCTTCAGGGATTAGCC TACTTACATTCTCATACTATGATTCATAGAGATATCAAAGCAGGAAATATCCTTCTGACAGAACCAGGCC AGGTGAAACTTGCTGACTTTGGCTCTGCTTCCATGGCATCACCTGCCAATTCCTTTGTGGGAACGCCGTA TTGGATGGCCCCAGAAGTAATTTTAGCCATGGATGAAGGACAATATGATGGCAAAGTAGATGTGTGGTCT CTTGGAATAACATGTATTGAACTAGCGGAAAGGAAGCCTCCTTTATTTAATATGAATGCAATGAGTGCCT TATATCACATAGCCCAAAATGAATCCCCTACACTACAGTCTAATGAATGGTCTGATTATTTTCGCAACTT TGTAGATTCTTGCCTCCAGAAAATCCCTCAAGATCGACCTACATCAGAGGAACTTTTAAAGCACATATTT GTTCTTCGGGAGCGCCCTGAAACCGTGTTAATAGATCTCATTCAGAGGACAAAGGATGCAGTAAGAGAGC TGGACAATCTGCAGTATCGAAAGATGAAGAAACTCCTTTTCCAGGAGGCACATAATGGACCAGCAGTAGA AGCACAGGAAGAAGAAGAGGAACAAGATCATGGTGTTGGCCGGACAGGAACAGTTAATAGTGTTGGAAGT AATCAATCCATTCCCAGCATGTCCATCAGTGCCAGCAGCCAAAGCAGTAGTGTTAACAGTCTTCCAGATG TCTCAGATGACAAGAGTGAGCTAGACATGATGGAGGGAGACCACACAGTGATGTCTAACAGTTCTGTTAT CCATTTAAAACCAGAGGAAGAAAATTACAGAGAAGAGGGAGATCCTAGAACAAGAGCATCAGATCCACAA TCTCCACCCCAAGTATCTCGTCACAAATCACACTATCGTAATCGAGAACACTTTGCTACTATACGGACAG CATCACTGGTTACGAGGCAAATGCAAGAACATGAGCAGGACTCTGAGCTTAGAGAACAAATGTCTGGCTA TAAGCGAATGAGGCGACAACATCAAAAGCAACTGATGACTCTGGAAAACAAGCTAAAGGCTGAGATGGAT GAACATCGCCTCAGATTAGACAAAGATCTTGAAACTCAGCGTAACAATTTTGCTGCAGAAATGGAGAAAC TTATCAAGAAACACCAGGCTGCCATGGAGAAAGAGGCTAAAGTGATGTCCAATGAAGAGAAAAAATTTCA GCAACATATTCAGGCCCAACAGAAGAAAGAACTGAATAGTTTTCTCGAGTCCCAGAAAAGAGAGTATAAA CTTCGAAAAGAGCAGCTTAAAGAGGAGCTAAATGAAAACCAGAGTACCCCCAAAAAAGAAAAACAGGAGT GGCTTTCAAAGCAGAAGGAGAATATACAGCATTTCCAAGCAGAAGAAGAAGCTAACCTTCTTCGACGTCA AAGACAATACCTAGAGCTGGAATGCCGTCGCTTCAAGAGAAGAATGTTACTTGGGCGTCATAACTTAGAG CAGGACCTTGTCAGGGAGGAGTTAAACAAAAGACAGACTCAGAAGGACTTAGAGCATGCCATGCTACTCC GACAGCATGAATCTATGCAAGAACTGGAGTTCCGCCACCTCAACACAATTCAGAAGATGCGCTGTGAGTT GATCAGATTACAGCATCAAACTGAGCTCACTAACCAGCTGGAATATAATAAGCGAAGAGAACGAGAACTA AGACGAAAGCATGTCATGGAAGTTCGACAACAGCCTAAGAGTTTGAAGTCTAAAGAACTCCAAATAAAAA AGCAGTTTCAGGATACCTGCAAAATCCAAACCAGACAGTACAAAGCATTAAGAAATCACCTGCTGGAGAC TACACCAAAGAGTGAGCACAAAGCTGTTCTGAAACGGCTCAAGGAGGAACAGACCCGGAAATTAGCTATC TTGGCTGAGCAGTATGATCACAGCATTAATGAAATGCTCTCCACACAAGCCCTGCGTTTGGATGAAGCAC AGGAAGCAGAGTGCCAGGTTTTGAAGATGCAGCTGCAGCAGGAACTGGAGCTGTTGAATGCGTATCAGAG CAAAATCAAGATGCAAGCTGAGGCACAACATGATCGAGAGCTTCGCGAGCTTGAACAGAGGGTCTCCCTC CGGAGGGCACTCTTAGAACAAAAGATTGAAGAAGAGATGTTGGCTTTGCAGAATGAGCGCACAGAACGAA TACGAAGCCTGTTGGAACGTCAAGCCAGAGAGATTGAAGCTTTTGACTCTGAAAGCATGAGACTAGGTTT TAGTAATATGGTCCTTTCTAATCTCTCCCCTGAGGCATTCAGCCACAGCTACCCGGGAGCTTCTGGTTGG TCACACAACCCTACTGGGGGTCCAGGACCTCACTGGGGTCATCCCATGGGTGGCCCACCACAAGCTTGGG GCCATCCAATGCAAGGTGGACCCCAGCCATGGGGTCACCCTTCAGGGCCAATGCAAGGGGTACCTCGAGG TAGCAGTATGGGAGTCCGCAATAGCCCCCAGGCTCTGAGGCGGACAGCTTCTGGGGGACGGACGGAGCAG GGCATGAGCAGAAGCACGAGTGTCACTTCACAAATATCCAATGGGTCACACATGTCTTATACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC219490 representing NM_020791 Red=Cloning site Green=Tags(s) MPSTNRAGSLKDPEIAELFFKEDPEKLFTDLREIGHGSFGAVYFARDVRTNEVVAIKKMSYSGKQSTEKW QDIIKEVKFLQRIKHPNSIEYKGCYLREHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAITHGALQGLA YLHSHTMIHRDIKAGNILLTEPGQVKLADFGSASMASPANSFVGTPYWMAPEVILAMDEGQYDGKVDVWS LGITCIELAERKPPLFNMNAMSALYHIAQNESPTLQSNEWSDYFRNFVDSCLQKIPQDRPTSEELLKHIF VLRERPETVLIDLIQRTKDAVRELDNLQYRKMKKLLFQEAHNGPAVEAQEEEEEQDHGVGRTGTVNSVGS NQSIPSMSISASSQSSSVNSLPDVSDDKSELDMMEGDHTVMSNSSVIHLKPEEENYREEGDPRTRASDPQ SPPQVSRHKSHYRNREHFATIRTASLVTRQMQEHEQDSELREQMSGYKRMRRQHQKQLMTLENKLKAEMD EHRLRLDKDLETQRNNFAAEMEKLIKKHQAAMEKEAKVMSNEEKKFQQHIQAQQKKELNSFLESQKREYK LRKEQLKEELNENQSTPKKEKQEWLSKQKENIQHFQAEEEANLLRRQRQYLELECRRFKRRMLLGRHNLE QDLVREELNKRQTQKDLEHAMLLRQHESMQELEFRHLNTIQKMRCELIRLQHQTELTNQLEYNKRREREL RRKHVMEVRQQPKSLKSKELQIKKQFQDTCKIQTRQYKALRNHLLETTPKSEHKAVLKRLKEEQTRKLAI LAEQYDHSINEMLSTQALRLDEAQEAECQVLKMQLQQELELLNAYQSKIKMQAEAQHDRELRELEQRVSL RRALLEQKIEEEMLALQNERTERIRSLLERQAREIEAFDSESMRLGFSNMVLSNLSPEAFSHSYPGASGW SHNPTGGPGPHWGHPMGGPPQAWGHPMQGGPQPWGHPSGPMQGVPRGSSMGVRNSPQALRRTASGGRTEQ GMSRSTSVTSQISNGSHMSYT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites1 | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_020791 |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| RefSeq | NM_020791.4 |
| RefSeq Size | 12096 bp |
| RefSeq ORF | 3006 bp |
| Locus ID | 57551 |
| UniProt ID | Q7L7X3 |
| Cytogenetics | 17q11.2 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | MAPK signaling pathway |
| Gene Summary | Serine/threonine-protein kinase involved in various processes such as p38/MAPK14 stress-activated MAPK cascade, DNA damage response and regulation of cytoskeleton stability. Phosphorylates MAP2K3, MAP2K6 and MARK2. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade by mediating phosphorylation and subsequent activation of the upstream MAP2K3 and MAP2K6 kinases. Involved in G-protein coupled receptor signaling to p38/MAPK14. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of MAP2K3 and MAP2K6. Acts as a regulator of cytoskeleton stability by phosphorylating 'Thr-208' of MARK2, leading to activate MARK2 kinase activity and subsequent phosphorylation and detachment of MAPT/TAU from microtubules. Also acts as a regulator of apoptosis: regulates apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation via activation of the MAPK8/JNK cascade.[UniProtKB/Swiss-Prot Function] |
| MW | 116.1 kDa |